Impact of Nano-Fe fertilizer rates on germination traits and protein pattern of three Mellisa offisinalis species

Document Type : Original Article

Authors

1 Assisstant Proff. Shahed University

2 College of Biology, Islamic Azad University, Islamshahr branch

Abstract

Genera of Lemon balm (Melissa officinalis L) and Moldavian dragonhead (Dracocephalum moldavica) belong to the Lamiaceae family. The plants due to its medicinal compounds, particularly essential oils; are very important for cultivation and quality improvement. The aim of this study was to investigate the effect of Nano-Fe fertilizer rates on seed germination indices.
The experiment was carried out based on factorial complete randomized design with two factors and three replicates. The factors were Nano-Fe with five levels and genetics of the plants with three levels. The results showed that different levels of Nano-Fe had high significant effect on germination rate, mean of germination day and root to shoot ratio among genetic accessions. The interaction was significant only in terms of root to shoot ratio. The Nano-Fe fertilizer significantly improved the root length. The highest germination rate was obtained in treatment of 8 mg/L and M. officinalis genus. The highest root to shoot ratio was achieved in 4 mg/L of Nano-Fe fertilizer and M. officinalis genus. According to the electrophoresis results of nine types of protein band of the molecular weight between 10 to 150 kDa among the genetic masses, three proteins were polymorphism. The increase of Nano-Fe fertilizer led to the small increase of total protein and 16 mg/L treatment had the highest protein content, while, no alteration was discovered in the protein quality (protein band type). Moreover, it was found that the mass of M. officinalis had a bit higher protein content in comparison to two D. moldavica ones.

Keywords

References

امید بیگی، ر. 1379. رهیافت­های تولید و فرآوری گیاهان دارویی. طراحان نشر. 379 صفحه.
پیوندی، م، پرنده، ه، و م . میرزاد، 1390. مقایسه تأثیر نانوکلات آهن با کلات آهن بر پارامترهای رشد و فعالیت آنزیم­های آنتی­اکسیدان ریحان (Ocimum basilicum) فصلنامه تازه­های بیوتکنولوژی سلولی مولکولی، شماره 4 ، صص89- 95.
تایز ل، زایگر ا، فیزیولوژی گیاهی، خانه زیست شناسی (ویرایش سوم) تهران، 2002، صفحه 780
خوشخوی، م. 1375. گیاه­افزایی (ازدیاد نباتات) مبانی و روش ها. (ترجمه)، جلد اول. انتشارات دانشگاه شیراز. ضفحه 373.
رحیم­زاده، س.، سهرابی، ی.، حیدری، غ م.، عیوضی، ع ر و حسینی، ط،1390. تاثیر کاربرد کودهای شیمیایی و زیستی بر عملکرد و درصد اسانس گیاه دارویی بادرشبو (Dracocephalum moldavica L.)، فصلنامه تحقیقات گیاهان دارویی و معطر ایران، شماره 1، صص 81-96.
زرگری، ع.، 1376. گیاهان دارویی (جلد چهارم). انتشارات دانشگاه تهران، تهران، 969 صفحه.
سلطانى، ابوالقاسم‏، دایرة­المعارف طب سنتى (گیاهان دارویى)، 1383، ارجمند، دانشگاه علوم پزشکى و خدمات درمانى‏، شماره 2، صص 524.
فرزانه، ه.، ١٣٦٩، آگروشیمی (ترجمه)، انتشارات آوای نور.
Agrawal RL. 2004. Seed Technology. New Dehli, Oxford IBH pub;pp:104-6.
Bajii, M., J.m. kinet., S. Lutts. 2002. Osmotic and ionic effects of nacl on germination,early seeding growth,and ion content of Atriplex halimus (Chenopodiaceae). Canadian Journal of Botany 80, 297-304.
Bavaresco, L., Fregoni, M. (1992). Investigation on some physiological parameters involved in chlorosis occurrence in grapevine. Plant Nutr, Vol, 15, PP. 1791-1807.
Bemier, G., Kinet, J M., and Sachs, R M. (1985). The physiology of flowering. CRC press. Inc., Flirida. PP. 13-20.
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry. 72:248-254.
De Villires, A J., Van Rooyrn, M W., Theron, G K., and Van Deventer, HA. (1994). Germination of three Namaqualand pioneer species, as in fluenced by salinity, temperature and light. Seed Science Technology 22: 427 – 433
Ellis RH, Roberts EH. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science Technology 9:373-409
Hogstrom, G R. (1984). Correct management practices for correcting iron deficiency in plants with emphasis on soil
Huntr, E A., Glasbey C A., and Naylov, R E L. (1984). The analysis of data from germination tests. Journal of Agricultural Science, Cambridge.102:207-213.
Klimek, B., Majda, T., and Patora, J. (1998). Investigation of essential oil and phenolic compounds of essential oil and phenolic compounds of lemon balm (Melissa officinalis L.) cultivated in Poland. Vth Conference on the Application of Chromatographic Methods in phytochemical and Biomedical Research Lublin, Poland. Herbpolonica .44: 4, 324- 331
Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970. 227(5259): p. 680-685.
Mazaherinia, S., Astaraei, A.R., Fotovat, A., Monshi, A., 2010. Nano iron oxide particles efficiency on Fe, Mn, Zn and Cu concentrations in wheat plant. World Appl. Sci. J. 7(1), 36- 40.  
 Morales, F., Grasa, R., Abadia A., and Abadia, J. (1998). Iron chlorosis paradox in fruit trees. Journal of Plant Nutrition. Vol. 21, PP. 815-825.

Mortvedt, J J. (1986). Iron sources and management practices for correcting iron chlorosis problems. Journal of Plant Nutrition, Vol. 9, PP. 961-974.

Radwan, S A., El-Koly, A S., and Sammaour, R H. (2013). Genetic variation among accessions of Lathyrus inconspicuous (L.) as revealed by SDS Polyacrylamide Gel Electrophoresis, Acta agriculturae Slovenica, 21 – 30, 1.01.
Talei, D., Mihdzar, A.K., Khanif, M.Y., Saad, M.S., Valdiani, A.R. (2011). Effects of different surface sterilizers on seed germination and contamination of king of bitters (Andrographis paniculata nees.). American-Eurasian journal of agricultural and environmental sciences 10:639-643.
Talei, D., Valdiani, A., Puad, M. (2013). An effective protein extraction method for two-dimensional electrophoresis in the anticancer herb (Andrographis paniculata Nees.). Biotechnology and Applied Biochemistry, 2013. doi: 10.1155/2013/319047.

Weitzel C, Petersen M. 2011. Cloning and characterization of rosmarinic acid synthase from Melissa officinalis L. Phytochemistry 72, 572 - 578

Horticultural Plants Nutrition
Volume 1, Issue 1 - Serial Number 1
September 2018
Pages 59-68

Files

Share

How to cite

Statistics